abstract：:In the present work, we propose the use of the Laser Scanning Confocal Microscopy (LSCM) to determine the effect of water repellents on rock's pore-network configuration and interconnection. The rocks studied are sandstones of Miocene age, a building material that is commonly found in the architectural heritage of Tun...

journal_title：Microscopy research and technique

authors： Zoghlami K,Gómez-Gras D,Corbella M,Darragi F

更新日期：2008-11-01 00:00:00

abstract：:We propose to cascade the Shape-Preserving Piecewise Cubic Hermite model with the Autoregressive Moving Average (ARMA) interpolator; we call this technique the Shape-Preserving Piecewise Cubic Hermite Autoregressive Moving Average (SP2CHARMA) model. In a few test cases involving different images, this model is found t...

journal_title：Microscopy research and technique

authors： Sim KS,Wee MY,Lim WK

更新日期：2008-10-01 00:00:00

abstract：:Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focu...

journal_title：Microscopy research and technique

authors： Punge A,Rizzoli SO,Jahn R,Wildanger JD,Meyer L,Schönle A,Kastrup L,Hell SW

更新日期：2008-09-01 00:00:00

abstract：:We measured cell membrane deformations under the modulation of piconewton magnetic force by using optical tracking and noninterferometric widefield optical profilometry. The magnetic force was applied to fibronectin-coated paramagnetic beads that bound to transmembrane protein integrins. At an image-acquisition rate o...

journal_title：Microscopy research and technique

authors： Wang CC,Jian HJ,Wu CW,Lee CH

更新日期：2008-08-01 00:00:00

abstract：:The applicability of the binomial frequency distribution is outlined for the analysis of the evolution nanoscale atomic clustering of dilute solute in an alloy subject to thermal ageing in 3D atom probe data. The conventional chi(2) statistics and significance testing are demonstrated to be inappropriate for compariso...

journal_title：Microscopy research and technique

authors： Moody MP,Stephenson LT,Ceguerra AV,Ringer SP

更新日期：2008-07-01 00:00:00

abstract：:The objectives were to develop method of isolating viable human limbal basal cells in order to enrich a subset of small cells with a large Nucleus/Cytoplasm (N/C) ratio expressing high levels of p63, nuclear protein. Limbal tissues were treated with trypsin for 50 min at 37 degrees C in an orbital shaker at 100 rpm wi...

journal_title：Microscopy research and technique

authors： Arpitha P,Prajna NV,Srinivasan M,Muthukkaruppan V

更新日期：2008-06-01 00:00:00

abstract：:Hybrid materials of polyacrylamide networks and gold nanoparticles were prepared by directly heating an aqueous solution containing HAuCl(4), acrylamide, N,N'-methylenebis-acrylamide, and sodium sulfite (Na(2)SO(3)). Acrylamide, N,N'-methylenebis-acrylamide, and Na(2)SO(3) were used as monomers, crosslinking agent, an...

journal_title：Microscopy research and technique

authors： Song Y,Li Z,Wang L,Yao Y,Chen C,Cui K

更新日期：2008-06-01 00:00:00

abstract：:Laminins are alpha-beta-gamma heterotrimeric components of all basement membranes. Laminins are now known to play the central role in organizing and establishing the basement membrane. The diversity of laminins allows them to impart special structural and signaling properties to the basement membrane. Of the 12 known ...

journal_title：Microscopy research and technique

authors： Miner JH

更新日期：2008-05-01 00:00:00

abstract：:Different methods have been established for the simultaneous detection of different pathogens in tissue samples, each with certain advantages and disadvantages. Chromogenic in situ hybridization combines specific molecular pathogen detection with microscopic evaluation of pathogen quantity, morphology and distribution...

journal_title：Microscopy research and technique

authors： Richter B,Fragner K,Weissenböck H

更新日期：2008-04-01 00:00:00

abstract：:Scanning electron microscope (SEM) investigations on the holdfast elements, proboscis hooks, and trunk spines of Dentitruncus truttae (Acanthocephala, Palaeacanthocephala), an endoparasite of Salmo trutta (brown trout), provide more data about the surface of these taxonomic relevant structures. In both acanthocephalan...

journal_title：Microscopy research and technique

authors： Dezfuli BS,Lui A,Giari L,Boldrini P,Giovinazzo G

更新日期：2008-03-01 00:00:00

abstract：:We combine reflective confocal microscopy with multiphoton microscopy to form a minimally invasive technique to observe the cornea. The two imaging modalities allow detection of complementary information from the cornea. The autofluorescence signal shows the cytoplasm of epithelial cells, and the second harmonic gener...

journal_title：Microscopy research and technique

authors： Chen WL,Sun Y,Lo W,Tan HY,Dong CY

更新日期：2008-02-01 00:00:00

abstract：:Biodegradable microspheres such as those made of poly-D,L-lactide (PDLLA) are widely investigated delivery systems for drugs or antigens. The aim of this study was to examine experimental conditions in order to produce PDLLA microspheres with the best properties for controlled and sustained drug delivery by the modifi...

journal_title：Microscopy research and technique

authors： Jovanović I,Jordović B,Petković M,Ignjatović N,Uskoković D

更新日期：2008-02-01 00:00:00

abstract：:In this study we report for the first time the localization of a photoreceptor pigment in the cilia of the colored heterotrich ciliates Blepharisma japonicum red and blue form, Fabrea salina, and Stentor coeruleus, as result of a confocal microscopy investigation. Optical sectioning confocal microscopy has been used f...

journal_title：Microscopy research and technique

authors： Colombetti G,Checcucci G,Lucia S,Usai C,Ramoino P,Bianchini P,Pesce M,Vicidomini G,Diaspro A

更新日期：2007-12-01 00:00:00

abstract：:Focused ion beam (FIB) techniques are among the most important tools for the nanostructuring of surfaces. We used the FIB/SEM (scanning electron microscope) for milling and imaging of digestive gland cells. The aim of our study was to document the interactions of FIB with the surface of the biological sample during FI...

journal_title：Microscopy research and technique

authors： Drobne D,Milani M,Leser V,Tatti F

更新日期：2007-10-01 00:00:00

abstract：:We have in recent studies presented unexpected immunohistochemical evidence favoring the existence of a local production of catecholamines, and an occurrence of adrenergic receptors on the tendon cells (tenocytes), in the human patellar tendon. This was particularly noticed for tendons from patients suffering from ten...

journal_title：Microscopy research and technique

authors： Danielson P,Alfredson H,Forsgren S

更新日期：2007-10-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) has become an increasingly important measurement tool for biological and biomedical investigations, with the capability to assay molecular dynamics and interactions both in vitro and within living cells. Information recovery in FCS requires an accurate characterization and c...

journal_title：Microscopy research and technique

authors： Wu J,Berland K

更新日期：2007-08-01 00:00:00

abstract：:The purpose of this in vitro study was to evaluate the interaction pattern of adhesive systems on laser and bur cavities. Cavities were prepared according to the following groups (n=9): (G1) conventional diamond bur (No. 1013); (G2) Er:YAG laser (250 mJ, 4 Hz, 80.6 J/cm2); (G3) Er,Cr:YSGG laser (3.5 W, 20 Hz, 61.7 J/c...

journal_title：Microscopy research and technique

authors： Aranha AC,De Paula Eduardo C,Gutknecht N,Marques MM,Ramalho KM,Apel C

更新日期：2007-08-01 00:00:00

abstract：:The wavelength of Raman-scattered light depends on the molecular composition of the substance. This is the first attempt to acquire Raman spectra of a mouse eyeball removed from a living mouse, in which the eyeball was preserved using the "in vivo cryotechnique" followed by freeze-drying. Eyeballs were cryofixed using...

journal_title：Microscopy research and technique

authors： Terada N,Ohno N,Saitoh S,Fujii Y,Ohguro H,Ohno S

更新日期：2007-07-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is a very sensitive technique that can be used, e.g., for the measurement of low concentrations and for the investigation of transport of fluorescent molecules. Fluorescence lifetime imaging (FLIM) provides spatially resolved information about molecular fluorescence lifetime...

journal_title：Microscopy research and technique

authors： Petrásek Z,Krishnan M,Mönch I,Schwille P

更新日期：2007-05-01 00:00:00

abstract：:High speed data registration is required for the study of fluorescence resonance energy transfer in real time as well as fast dynamic intra- and inter-cellular signaling events. Multispectral confocal spinning disk microscopy provides a high resolution method for performing such real time live cell imaging. However, o...

journal_title：Microscopy research and technique

authors： Perrine KA,Lamarche BL,Hopkins DF,Budge SE,Opresko LK,Wiley HS,Sowa MB

更新日期：2007-04-01 00:00:00

abstract：:Correlative microscopy has become increasingly important for the analysis of the structure, function, and dynamics of cells. This is largely due to the result of recent advances in light-, probe-, laser- and various electron microscopy techniques that facilitate three-dimensional studies. Furthermore, the improved und...

journal_title：Microscopy research and technique

authors： Braet F,Wisse E,Bomans P,Frederik P,Geerts W,Koster A,Soon L,Ringer S

更新日期：2007-03-01 00:00:00

abstract：:In vivo and in vitro multiphoton imaging was used to perform high resolution optical sectioning of human hair by nonlinear excitation of endogenous as well as exogenous fluorophores. Multiphoton fluorescence lifetime imaging (FLIM) based on time-resolved single photon counting and near-infrared femtosecond laser pulse...

journal_title：Microscopy research and technique

authors： Ehlers A,Riemann I,Stark M,König K

更新日期：2007-02-01 00:00:00

abstract：:We present a semiautomated software solution to the problem of extending the lateral field of view of a classical microscope. The initial requirements are a set of overlapping images, along with their user-provided coarse mosaic. Our solution then refines this initial mosaic in a fully automatic fashion. We rely on a ...

journal_title：Microscopy research and technique

authors： Thévenaz P,Unser M

更新日期：2007-02-01 00:00:00

abstract：:Magnetotactic bacteria orient and migrate along geomagnetic field lines. Each cell contains membrane-enclosed, nano-scale, iron-mineral particles called magnetosomes that cause alignment of the cell in the geomagnetic field as the bacteria swim propelled by flagella. In this work we studied the ultrastructure of the f...

journal_title：Microscopy research and technique

authors： Silva KT,Abreu F,Almeida FP,Keim CN,Farina M,Lins U

更新日期：2007-01-01 00:00:00

abstract：:All termite species (Isoptera) are eusocial. These insects live in societies where the division of labor is a predominant characteristic, conditioned by the presence of castes with different morphology, ontogeny, and development. The soldier caste is unique among social insects and it is responsible for colony defense...

journal_title：Microscopy research and technique

authors： Santos CA,Costa-Leonardo AM

更新日期：2006-11-01 00:00:00

abstract：:A common feature in the configuration of germinal vesicle (GV) chromatin in most species is that diffuse chromatin condenses into a perinucleolar ring during follicular growth; however, no such ring was observed in goat oocytes. Reports on whether bovine GV chromatin condenses into a perinucleolar ring are controversi...

journal_title：Microscopy research and technique

authors： Liu Y,Sui HS,Wang HL,Yuan JH,Luo MJ,Xia P,Tan JH

更新日期：2006-10-01 00:00:00

abstract：:This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-mum thick CsI(Tl) scintillation crystal. The vi...

journal_title：Microscopy research and technique

authors： Jheon S,Youn HS,Kim HT,Choi GH,Kim JK

更新日期：2006-08-01 00:00:00

abstract：:In this paper, an application of contact microradiography with soft X-rays for detecting the uptake site of heavy metal in the whole plant leaves is investigated. The X-ray source is a laser-plasma one based on an Nd:glass laser. The soft X-ray radiation emitted from the plasma laser targets of magnesium, iron, and co...

journal_title：Microscopy research and technique

authors： Reale L,Lai A,Bellucci I,Faenov A,Pikuz T,Flora F,Spanò L,Poma A,Limongi T,Palladino L,Ritucci A,Tomassetti G,Petrocelli G,Martellucci S

更新日期：2006-08-01 00:00:00

abstract：:The mammalian zona pellucida (ZP) is an extracellular matrix surrounding oocytes and early embryos, which is critical for normal fertilization and preimplantation development. It is made up of three/four glycoproteins arranged in a delicate filamentous matrix. Scanning electron microscopy (SEM) studies have shown that...

journal_title：Microscopy research and technique

authors： Familiari G,Relucenti M,Heyn R,Micara G,Correr S

更新日期：2006-06-01 00:00:00

abstract：:The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitoc...

journal_title：Microscopy research and technique

authors： Sathananthan AH,Selvaraj K,Girijashankar ML,Ganesh V,Selvaraj P,Trounson AO

更新日期：2006-06-01 00:00:00

abstract：:The surface epithelial cells of the ovary, which are modified peritoneal cells, form a single, focally pseudostratified layer. The Müllerian ducts differentiate after invagination of the coelomic mesothelium over the gonadal ridges during the 6th week of embryonic life. On the basis of the embryologically putative Mül...

journal_title：Microscopy research and technique

authors： Okamura H,Katabuchi H,Nitta M,Ohtake H

更新日期：2006-06-01 00:00:00

abstract：:The imaging and characterization of dislocations is commonly carried out by thin foil transmission electron microscopy (TEM) using diffraction contrast imaging. However, the thin foil approach is limited by difficult sample preparation, thin foil artifacts, relatively small viewable areas, and constraints on carrying ...

journal_title：Microscopy research and technique

authors： Crimp MA

更新日期：2006-05-01 00:00:00

abstract：:The presence of hard jaws (trophi), with species-specific shape and size, is a taxonomic feature of Rotifera, a group of microscopic metazoans. Since trophi are used to discriminate among species, it is important to know whether these structures change in taxonomically important ways during postembryonic development. ...

journal_title：Microscopy research and technique

authors： Fontaneto D,Melone G

更新日期：2006-04-01 00:00:00

abstract：:A homemade colloidal probe atomic force microscope was used to perform nanoindentation with a spherical probe of 5 microm in diameter, at different approach velocities in order to extract the Young's modulus, E0, of poly(L-lysine)/hyaluronan (PLL/HA) films. This parameter is of prime importance to control cellular adh...

journal_title：Microscopy research and technique

authors： Francius G,Hemmerlé J,Ohayon J,Schaaf P,Voegel JC,Picart C,Senger B

更新日期：2006-02-01 00:00:00

abstract：:The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells ...

journal_title：Microscopy research and technique

authors： Carbone K,Pinto NM,Abrahamsohn PA,Zorn TM

更新日期：2006-01-01 00:00:00

abstract：:In the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae), there is on the dorsal side of the thorax, beneath the mesoscutum plate of the prothorax and around the median notal suture, a lump that, in the course of hornet activity, is warmer by 9 degrees C from the surrounding milieu and by up to 6 degrees C from...

journal_title：Microscopy research and technique

authors： Plotkin M,Ermakov NY,Volynchik S,Barkay Z,Bergman DJ,Ishay JS

更新日期：2005-12-15 00:00:00

abstract：:Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...

journal_title：Microscopy research and technique

authors： Szabo S,Haislip AM,Traina-Dorge V,Costin JM,Crawford BE 2nd,Wilson RB,Garry RF

更新日期：2005-11-01 00:00:00

abstract：:The Dominican Republic (DR) has experienced tremendous increase in the number of cases of dengue virus (DENV) reported in the past few years. There are four serotypes of DENV (1-4), and each can cause classic dengue fever (DF), dengue hemorrhagic fever (DHF), or dengue shock syndrome (DSS). DENV-1-4 are currently circ...

journal_title：Microscopy research and technique

authors： Cabrera-Batista B,Skewes-Ramm R,Fermin CD,Garry RF

更新日期：2005-11-01 00:00:00

abstract：:Photoconversion of fluorescent staining into stable diaminobenzidine (DAB) precipitate is widely used for neuroanatomical and developmental studies. An important advantage of the approach is to make correlations between light and electron microscopy analyses possible, the DAB reaction product formed during photoconver...

journal_title：Microscopy research and technique

authors： Nikonenko I,Boda B,Alberi S,Muller D

更新日期：2005-10-01 00:00:00

abstract：:The thin layer of liquid that lines the conducting airway epithelium, the airway surface liquid (ASL), is important for mucociliary clearance. Altered ionic composition and/ or volume of the ASL play a major role in the pathology of airway diseases such as cystic fibrosis. Since the ASL is a thin layer, it has been di...

journal_title：Microscopy research and technique

authors： Vanthanouvong V,Kozlova I,Roomans GM

更新日期：2005-09-01 00:00:00